An Indocyanine Green-Based Nanoprobe for In Vivo Detection of Cellular Senescence.
Andrew G BakerMuhamad HartonoHui-Ling OuAndrea Bistrović PopovEmma L BrownJames JosephMonika GolinskaEstela González-GualdaDavid MaciasJianfeng GeMary DenholmSamir MorsliChandan SangheraThomas R ElseHeather F GreerAude VernetSarah E BohndiekDaniel Muñoz-EspínLjiljana FrukPublished in: Angewandte Chemie (International ed. in English) (2024)
There is an urgent need to improve conventional cancer-treatments by preventing detrimental side effects, cancer recurrence and metastases. Recent studies have shown that presence of senescent cells in tissues treated with chemo- or radiotherapy can be used to predict the effectiveness of cancer treatment. However, although the accumulation of senescent cells is one of the hallmarks of cancer, surprisingly little progress has been made in development of strategies for their detection in vivo. To address a lack of detection tools, we developed a biocompatible, injectable organic nanoprobe (NanoJagg), which is selectively taken up by senescent cells and accumulates in the lysosomes. The NanoJagg probe is obtained by self-assembly of indocyanine green (ICG) dimers using a scalable manufacturing process and characterized by a unique spectral signature suitable for both photoacoustic tomography (PAT) and fluorescence imaging. In vitro, ex vivo and in vivo studies all indicate that NanoJaggs are a clinically translatable probe for detection of senescence and their PAT signal makes them suitable for longitudinal monitoring of the senescence burden in solid tumors after chemotherapy or radiotherapy.
Keyphrases
- fluorescence imaging
- induced apoptosis
- papillary thyroid
- cell cycle arrest
- loop mediated isothermal amplification
- early stage
- label free
- locally advanced
- endothelial cells
- photodynamic therapy
- living cells
- dna damage
- squamous cell
- randomized controlled trial
- radiation therapy
- endoplasmic reticulum stress
- systematic review
- stress induced
- gene expression
- cell death
- radiation induced
- optical coherence tomography
- signaling pathway
- magnetic resonance imaging
- lymph node metastasis
- computed tomography
- rectal cancer
- young adults
- cross sectional
- pi k akt
- combination therapy
- cancer therapy