Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression.
Pan GaoQing LyuAmr R GhanamCicera R LazzarottoGregory A NewbyWei ZhangMihyun ChoiOrazio J SlivanoKevin HoldenJohn A WalkerAnastasia P KadinaRob J MunroeChristian M AbratteJohn C SchimentiDavid R LiuShengdar Q TsaiXiaochun LongJoseph M MianoPublished in: Genome biology (2021)
PE2 directs high-fidelity editing of a single base in a TFBS leading to cell-specific loss in expression of an mRNA/long noncoding RNA gene pair. The PE2 platform expands the genome editing toolbox for modeling and correcting relevant noncoding SNVs in the mouse.
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