Extracellular Matrix Mimicking Wound Microenvironment Responsive Amyloid-Heparin@TA AgNP Co-Assembled Hydrogel: An Effective Conductive Antibacterial Wound Healing Material.
Nabanita MukherjeeSatyajit GhoshRajsekhar RoyDipro MukherjeeSamya SenDebasmita NandiJayita SarkarSurajit GhoshPublished in: ACS applied materials & interfaces (2024)
Bioengineered composite hydrogel platforms made of a supramolecular coassembly have recently garnered significant attention as promising biomaterial-based healthcare therapeutics. The mechanical durability of amyloids, in conjunction with the structured charged framework rendered by biologically abundant key ECM component glycosaminoglycan, enables us to design minimalistic customized biomaterial suited for stimuli responsive therapy. In this study, by harnessing the heparin sulfate-binding aptitude of amyloid fibrils, we have constructed a pH-responsive extracellular matrix (ECM) mimicking hydrogel matrix. This effective biocompatible platform comprising heparin sulfate-amyloid coassembled hydrogel embedded with polyphenol functionalized silver nanoparticles not only provide a native skin ECM-like conductive environment but also provide wound-microenvironment responsive on-demand superior antibacterial efficacy for effective diabetic wound healing. Interestingly, both the cytocompatibility and antibacterial properties of this bioinspired matrix can be fine-tuned by controlling the mutual ratio of heparin sulfate-amyloid and incubated silver nanoparticle components, respectively. The designed biomaterial platform exhibits notable effectiveness in the treatment of chronic hyperglycemic wounds infected with multidrug-resistant bacteria, because of the integration of pH-responsive release characteristics of the incubated functionalized AgNP and the antibacterial amyloid fibrils. In addition to this, the aforementioned assemblage shows exceptional hemocompatibility with significant antibiofilm and antioxidant characteristics. Histological evidence of the incised skin tissue sections indicates that the fabricated composite hydrogel is also effective in controlling pro-inflammatory cytokines such as IL6 and TNFα expressions at the wound vicinity with significant upregulation of angiogenesis markers like CD31 and α-SMA.
Keyphrases
- wound healing
- extracellular matrix
- silver nanoparticles
- venous thromboembolism
- healthcare
- multidrug resistant
- tissue engineering
- growth factor
- stem cells
- cancer therapy
- anti inflammatory
- quantum dots
- randomized controlled trial
- small molecule
- drug delivery
- oxidative stress
- poor prognosis
- high throughput
- big data
- wastewater treatment
- reduced graphene oxide
- mass spectrometry
- drug resistant
- cystic fibrosis
- working memory
- transcription factor
- bone marrow
- replacement therapy
- surgical site infection
- mesenchymal stem cells
- drug release