Molecular Cloning and Characterization of Small Heat Shock Protein Genes in the Invasive Leaf Miner Fly, Liriomyza trifolii.
Ya-Wen ChangXiao-Xiang ZhangMing-Xing LuYu-Zhou DuKeyan Zhu-SalzmanPublished in: Genes (2019)
Small heat shock proteins (sHSPs) comprise numerous proteins with diverse structure and function. As molecular chaperones, they play essential roles in various biological processes, especially under thermal stresses. In this study, we identified three sHSP-encoding genes, LtHSP19.5, LtHSP20.8 and LtHSP21.7b from Liriomyza trifolii, an important insect pest of ornamental and vegetable crops worldwide. Putative proteins encoded by these genes all contain a conserved α-crystallin domain that is typical of the sHSP family. Their expression patterns during temperature stresses and at different insect development stages were studied by reverse-transcription quantitative PCR (RT-qPCR). In addition, the expression patterns were compared with those of LtHSP21.3 and LtHSP21.7, two previously published sHSPs. When pupae were exposed to temperatures ranging from -20 to 45 °C for 1 h, all LtsHSPs were strongly induced by either heat or cold stresses, but the magnitude was lower under the low temperature range than high temperatures. Developmentally regulated differential expression was also detected, with pupae and prepupae featuring the highest expression of sHSPs. Results suggest that LtsHSPs play a role in the development of the invasive leaf miner fly and may facilitate insect adaptation to climate change.
Keyphrases
- heat shock
- heat shock protein
- poor prognosis
- climate change
- genome wide
- heat stress
- transcription factor
- genome wide identification
- aedes aegypti
- randomized controlled trial
- bioinformatics analysis
- binding protein
- long non coding rna
- gene expression
- single molecule
- oxidative stress
- genome wide analysis
- risk assessment
- mass spectrometry
- zika virus