Redox driven B 12 -ligand switch drives CarH photoresponse.

CarH is a coenzyme B 12 -dependent photoreceptor involved in regulating carotenoid biosynthesis. How light-triggered cleavage of the B 12 Co-C bond culminates in CarH tetramer dissociation to initiate transcription remains unclear. Here, a series of crystal structures of the CarH B 12 -binding domain after illumination suggest formation of unforeseen intermediate states prior to tetramer dissociation. Unexpectedly, in the absence of oxygen, Co-C bond cleavage is followed by reorientation of the corrin ring and a switch from a lower to upper histidine-Co ligation, corresponding to a pentacoordinate state. Under aerobic conditions, rapid flash-cooling of crystals prior to deterioration upon illumination confirm a similar B 12 -ligand switch occurs. Removal of the upper His-ligating residue prevents monomer formation upon illumination. Combined with detailed solution spectroscopy and computational studies, these data demonstrate the CarH photoresponse integrates B 12 photo- and redox-chemistry to drive large-scale conformational changes through stepwise Co-ligation changes.