A Peptide YGDEY from Tilapia Gelatin Hydrolysates Inhibits UVB-mediated Skin Photoaging by Regulating MMP-1 and MMP-9 Expression in HaCaT Cells.
Zhenbang XiaoPeng LiangJiali ChenMei-Fang ChenFang GongChengyong LiChunxia ZhouPengzhi HongPing YangZhong-Ji QianPublished in: Photochemistry and photobiology (2019)
In this study, we investigated the protective effects of a peptide (YGDEY, Tyr-Gly-Asp-Glu-Tyr) isolated from tilapia skin gelatin hydrolysates (TGHs), against UVB-induced photoaging in human keratinocytes (HaCaT) cells. Results showed that YGDEY significantly decreased levels of intracellular reactive oxygen species (ROS), increased antioxidant factors (Superoxide Dismutase, SOD and Glutathione, GSH) expression and maintained balance between GSH and GSSG in HaCaT cells. Comet assay shows that YGDEY can protect DNA from oxidative damage. Furthermore, it significantly inhibited MMP-1 (collagenase) and MMP-9 (gelatinase) expression and increased Type I procollagen production. In addition, the molecular docking study showed that YGDEY may form active sites with MMP-1 and MMP-9. Moreover, Western blot analysis was utilized to measure the protein levels of UVB-induced mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) signaling pathways. Therefore, these results suggested that YGDEY has a therapeutic effectiveness in prevention of UVB-induced cellular damage, and it is a candidate worthy of being developed as a potential natural antioxidant and food additive.
Keyphrases
- nuclear factor
- induced apoptosis
- oxidative stress
- signaling pathway
- reactive oxygen species
- diabetic rats
- molecular docking
- cell cycle arrest
- poor prognosis
- cell migration
- high glucose
- pi k akt
- endothelial cells
- toll like receptor
- binding protein
- systematic review
- randomized controlled trial
- cell death
- immune response
- high throughput
- drug induced
- risk assessment
- molecular dynamics simulations
- cell free
- south africa
- circulating tumor
- high resolution
- soft tissue
- epithelial mesenchymal transition
- small molecule
- protein kinase