Genome engineering in rodents - status quo and perspectives.

Published in: Laboratory animals (2021)

The introduction of the CRISPR-Cas9 system in 2013 has revolutionized experimental genetics in mice and rats. This commentary gives an overview on the use of CRISPR either for gene editing in the germline or for editing and beyond editing in somatic cells. Future perspectives are opened by emerging CRISPR technologies that could enable genome engineering at larger scale.

Keyphrases

crispr cas
genome editing
induced apoptosis
genome wide
cell cycle arrest
copy number
dna repair
endoplasmic reticulum stress
oxidative stress
dna methylation
skeletal muscle
cell proliferation