CRISPR-Cas9 delivery strategies and applications: Review and update.
Ali Alizadeh SeveriBahman AkbariPublished in: Genesis (New York, N.Y. : 2000) (2024)
Nowadays, a significant part of the investigations carried out in the medical field belong to cancer treatment. Generally, conventional cancer treatments, including chemotherapy, radiotherapy, and surgery, which have been used for a long time, are not sufficient, especially in malignant cancers. Because genetic mutations cause cancers, researchers are trying to treat these diseases using genetic engineering tools. One of them is clustered regularly interspaced short palindromic repeats (CRISPR), a powerful tool in genetic engineering in the last decade. CRISPR, which forms the CRISPR-Cas structure with its endonuclease protein, Cas, is known as a part of the immune system (adaptive immunity) in bacteria and archaea. Among the types of Cas proteins, Cas9 endonuclease has been used in many scientific studies due to its high accuracy and efficiency. This review reviews the CRISPR system, focusing on the history, classification, delivery methods, applications, new generations, and challenges of CRISPR-Cas9 technology.
Keyphrases
- crispr cas
- genome editing
- genome wide
- locally advanced
- copy number
- minimally invasive
- machine learning
- papillary thyroid
- deep learning
- dna methylation
- radiation therapy
- coronary artery bypass
- squamous cell carcinoma
- randomized controlled trial
- childhood cancer
- radiation induced
- amino acid
- gene expression
- percutaneous coronary intervention
- oxidative stress
- rectal cancer
- coronary artery disease
- lymph node metastasis
- binding protein