Arginine Methyltransferase PRMT7 Deregulates Expression of RUNX1 Target Genes in T-Cell Acute Lymphoblastic Leukemia.
Laura OksaArtturi MäkinenAtte NikkiläNoora HyvärinenSaara LaukkanenAnne RokkaPekka HaapaniemiMasafumi SekiJunko TakitaOtto KaukoMerja HeinäniemiOlli LohiPublished in: Cancers (2022)
T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy with no well-established prognostic biomarkers. We examined the expression of protein arginine methyltransferases across hematological malignancies and discovered high levels of PRMT7 mRNA in T-ALL, particularly in the mature subtypes of T-ALL. The genetic deletion of PRMT7 by CRISPR-Cas9 reduced the colony formation of T-ALL cells and changed arginine monomethylation patterns in protein complexes associated with the RNA and DNA processing and the T-ALL pathogenesis. Among them was RUNX1, whose target gene expression was consequently deregulated. These results suggest that PRMT7 plays an active role in the pathogenesis of T-ALL.
Keyphrases
- acute lymphoblastic leukemia
- binding protein
- nitric oxide
- gene expression
- amino acid
- crispr cas
- poor prognosis
- allogeneic hematopoietic stem cell transplantation
- induced apoptosis
- genome wide
- transcription factor
- genome editing
- dna methylation
- cell cycle arrest
- single molecule
- nucleic acid
- long non coding rna
- cell free
- signaling pathway
- copy number
- oxidative stress
- cell death
- genome wide analysis