Multivariate Analysis of Difference Raman Spectra of the Irradiated Nucleus and Cytoplasm Region of SH-SY5Y Human Neuroblastoma Cells.
Ines DelfinoValerio RicciardiLorenzo MantiMaria LasalviaMaria LeporePublished in: Sensors (Basel, Switzerland) (2019)
Previous works showed that spatially resolved Raman spectra of cytoplasm and nucleus region of single cells exposed to X-rays evidence different features. The present work aims to introduce a new approach to profit from these differences to deeper investigate X-ray irradiation effects on single SH-SY5Y human neuroblastoma cells. For this aim, Raman micro-spectroscopy was performed in vitro on single cells after irradiation by graded X-ray doses (2, 4, 6, 8 Gy). Spectra from nucleus and cytoplasm regions were selectively acquired. The examination by interval Principal Component Analysis (i-PCA) of the difference spectra obtained by subtracting each cytoplasm-related spectrum from the corresponding one detected at the nucleus enabled us to reveal the subtle modifications of Raman features specific of different spatial cell regions. They were discussed in terms of effects induced by X-ray irradiation on DNA/RNA, lipids, and proteins. The proposed approach enabled us to evidence some features not outlined in previous investigations.
Keyphrases
- induced apoptosis
- cell cycle arrest
- endothelial cells
- endoplasmic reticulum stress
- density functional theory
- stem cells
- computed tomography
- signaling pathway
- gene expression
- cell death
- oxidative stress
- magnetic resonance
- mesenchymal stem cells
- dna methylation
- dual energy
- fatty acid
- mass spectrometry
- circulating tumor cells
- pluripotent stem cells