Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis.

Aimee A MalzahnXu TangKeunsub LeeQiurong RenSimon SretenovicYingxiao ZhangHongqiao ChenMinjeong KangYu BaoXuelian ZhengKejun DengTao ZhangValeria SalcedoKan WangYong ZhangYiping Qi

Published in: BMC biology (2019)

Our study demonstrates the use of high-temperature regimes to achieve high editing efficiencies with Cas12a systems in rice, Arabidopsis, and maize and sheds light on the mechanism of temperature sensitivity for Cas12a in plants.

Keyphrases

crispr cas
genome editing
high temperature
transcription factor
cell wall
plant growth