Prospective isolation of chondroprogenitors from human iPSCs based on cell surface markers identified using a CRISPR-Cas9-generated reporter.

Amanda DicksChia-Lung WuNancy StewardShaunak S AdkarCharles A GersbachFarshid Guilak

Published in: Stem cell research & therapy (2020)

Overall, this study has identified a unique hiPSC-derived subpopulation of chondroprogenitors that are CD146+/CD166+/PDGFRβ+/CD45- and exhibit high chondrogenic potential, providing a purified cell source for cartilage tissue engineering or disease modeling studies.

Keyphrases

crispr cas
tissue engineering
cell surface
genome editing
induced pluripotent stem cells
endothelial cells
single cell
mesenchymal stem cells
cell therapy
pluripotent stem cells
stem cells
case control
human health
climate change