miR-202-5p Inhibits Lipid Metabolism and Steroidogenesis of Goose Hierarchical Granulosa Cells by Targeting ACSL3.
Mingxia RanShenqiang HuQingyuan OuyangHengli XieXi ZhangYueyue LinXuejian LiJiwei HuLiang LiHua HeHehe LiuJiwen WangPublished in: Animals : an open access journal from MDPI (2023)
miRNAs are critical for steroidogenesis in granulosa cells (GCs) during ovarian follicular development. We have previously shown that miR-202-5p displays a stage-dependent expression pattern in GCs from goose follicles of different sizes, suggesting that this miRNA could be involved in the regulation of the functions of goose GCs; therefore, in this study, the effects of miR-202-5p on lipid metabolism and steroidogenesis in goose hierarchical follicular GCs (hGCs), as well as its mechanisms of action, were evaluated. Oil Red O staining and analyses of intracellular cholesterol and triglyceride contents showed that the overexpression of miR-202-5p significantly inhibited lipid deposition in hGCs; additionally, miR-202-5p significantly inhibited progesterone secretion in hGCs. A bioinformatics analysis and luciferase reporter assay indicated that Acyl-CoA synthetase long-chain family member 3 ( ACSL3 ), which activates long-chain fatty acids for the synthesis of cellular lipids, is a potential target of miR-202-5p. ACSL3 silencing inhibited lipid deposition and estrogen secretion in hGCs. These data suggest that miR-202-5p exerts inhibitory effects on lipid deposition and steroidogenesis in goose hGCs by targeting the ACSL3 gene.
Keyphrases
- fatty acid
- bioinformatics analysis
- induced apoptosis
- poor prognosis
- cell proliferation
- polycystic ovary syndrome
- estrogen receptor
- high throughput
- crispr cas
- copy number
- oxidative stress
- electronic health record
- cell death
- risk assessment
- machine learning
- flow cytometry
- transcription factor
- signaling pathway
- skeletal muscle
- metabolic syndrome
- insulin resistance
- genome wide analysis