A field survey using LAMP assay for detection of Schistosoma mansoni in a low-transmission area of schistosomiasis in Umbuzeiro, Brazil: Assessment in human and snail samples.

Javier GandaseguiPedro Fernández-SotoAntonio MuroConstança Simões BarbosaFabio Lopes de MeloRodrigo LoyoElainne Christine de Souza Gomes

Published in: PLoS neglected tropical diseases (2018)

This is the first study in which a LAMP assay was evaluated in both human stool and snail samples from a low-transmission schistosomiasis-endemic area. Our SmMIT-LAMP proved to be much more efficient in detection of S. mansoni in comparison to the 'gold standard' Kato-Katz method in human stool samples and the reference molecular nested-PCR in snails. The SmMIT-LAMP has demonstrated to be a useful molecular tool to identify potential foci of transmission in order to build risk maps of schistosomiasis.

Keyphrases

loop mediated isothermal amplification
endothelial cells
induced pluripotent stem cells
epithelial mesenchymal transition
pluripotent stem cells
sensitive detection
high throughput
cross sectional
climate change
single cell
human health