Future of human mitochondrial DNA editing technologies.
N VerechshaginaN NikitchinaY YamadaН HarashimaM TanakaKonstantin E OrishchenkoIlya MazuninPublished in: Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis (2018)
ATP and other metabolites, which are necessary for the development, maintenance, and functioning of bodily cells are all synthesized in the mitochondria. Multiple copies of the genome, present within the mitochondria, together with its maternal inheritance, determine the clinical manifestation and spreading of mutations in mitochondrial DNA (mtDNA). The main obstacle in the way of thorough understanding of mitochondrial biology and the development of gene therapy methods for mitochondrial diseases is the absence of systems that allow to directly change mtDNA sequence. Here, we discuss existing methods of manipulating the level of mtDNA heteroplasmy, as well as the latest systems, that could be used in the future as tools for human mitochondrial genome editing.
Keyphrases
- mitochondrial dna
- copy number
- crispr cas
- genome editing
- endothelial cells
- gene therapy
- oxidative stress
- cell death
- genome wide
- induced apoptosis
- induced pluripotent stem cells
- current status
- pluripotent stem cells
- cell cycle arrest
- reactive oxygen species
- ms ms
- physical activity
- endoplasmic reticulum
- body mass index
- cell proliferation
- pregnant women
- weight gain