Foodborne pathogenic infection has brought multifaceted issues to human life, leading to an urgent demand for advanced detection technologies. CRISPR/Cas-based biosensors have the potential to address various challenges that exist in conventional assays such as insensitivity, long turnaround time and complex pretreatments. In this perspective, we review the relevant strategies of CRISPR/Cas-assisted diagnostics on foodborne pathogens, focusing on biosensing platforms for foodborne pathogens based on fluorescence, colorimetric, (electro)chemiluminescence, electrochemical, and surface-enhanced Raman scattering detection. It summarizes their detection principles by the clarification of foodborne pathogenic bacteria, fungi, and viruses. Finally, we discuss the current challenges or technical barriers of these methods against broad application, and put forward alternative solutions to improve CRISPR/Cas potential for food safety.
Keyphrases
- crispr cas
- genome editing
- label free
- loop mediated isothermal amplification
- gram negative
- gold nanoparticles
- real time pcr
- antimicrobial resistance
- sensitive detection
- endothelial cells
- human health
- single molecule
- ionic liquid
- risk assessment
- multidrug resistant
- hydrogen peroxide
- living cells
- nitric oxide
- induced pluripotent stem cells
- single cell
- fluorescent probe
- mass spectrometry