The Regulatory Network of Sweet Corn ( Zea mays L.) Seedlings under Heat Stress Revealed by Transcriptome and Metabolome Analysis.
Zhuqing WangYang XiaoHailong ChangShengren SunJianqiang WangQinggan LiangQingdan WuJiantao WuYuanxia QinJunlv ChenGang WangQinnan WangPublished in: International journal of molecular sciences (2023)
Heat stress is an increasingly significant abiotic stress factor affecting crop yield and quality. This study aims to uncover the regulatory mechanism of sweet corn response to heat stress by integrating transcriptome and metabolome analyses of seedlings exposed to normal (25 °C) or high temperature (42 °C). The transcriptome results revealed numerous pathways affected by heat stress, especially those related to phenylpropanoid processes and photosynthesis, with 102 and 107 differentially expressed genes (DEGs) identified, respectively, and mostly down-regulated in expression. The metabolome results showed that 12 or 24 h of heat stress significantly affected the abundance of metabolites, with 61 metabolites detected after 12 h and 111 after 24 h, of which 42 metabolites were detected at both time points, including various alkaloids and flavonoids. Scopoletin-7-o-glucoside (scopolin), 3-indolepropionic acid, acetryptine, 5,7-dihydroxy-3',4',5'-trimethoxyflavone, and 5,6,7,4'-tetramethoxyflavanone expression levels were mostly up-regulated. A regulatory network was built by analyzing the correlations between gene modules and metabolites, and four hub genes in sweet corn seedlings under heat stress were identified: RNA-dependent RNA polymerase 2 ( RDR2 ), UDP-glucosyltransferase 73C5 ( UGT73C5 ), LOC103633555 , and CTC-interacting domain 7 ( CID7 ). These results provide a foundation for improving sweet corn development through biological intervention or genome-level modulation.
Keyphrases
- heat stress
- genome wide
- heat shock
- transcription factor
- ms ms
- single cell
- genome wide identification
- poor prognosis
- rna seq
- gene expression
- dna methylation
- randomized controlled trial
- arabidopsis thaliana
- high temperature
- network analysis
- copy number
- climate change
- bioinformatics analysis
- oxidative stress
- long non coding rna
- circulating tumor cells
- genome wide analysis
- stress induced
- quality improvement