From Bugs to Bioplastics: Total (+)-Dihydrocarvide Biosynthesis by Engineered Escherichia coli.
Gabriel A Ascue AvalosHelen S ToogoodShirley TaitHanan L MessihaNigel S ScruttonPublished in: Chembiochem : a European journal of chemical biology (2019)
The monoterpenoid lactone derivative (+)-dihydrocarvide ((+)-DHCD) can be polymerised to form shape-memory polymers. Synthetic biology routes from simple, inexpensive carbon sources are an attractive, alternative route over chemical synthesis from (R)-carvone. We have demonstrated a proof-of-principle in vivo approach for the complete biosynthesis of (+)-DHCD from glucose in Escherichia coli (6.6 mg L-1 ). The pathway is based on the Mentha spicata route to (R)-carvone, with the addition of an 'ene'-reductase and Baeyer-Villiger cyclohexanone monooxygenase. Co-expression with a limonene synthesis pathway enzyme enables complete biocatalytic production within one microbial chassis. (+)-DHCD was successfully produced by screening multiple homologues of the pathway genes, combined with expression optimisation by selective promoter and/or ribosomal binding-site screening. This study demonstrates the potential application of synthetic biology approaches in the development of truly sustainable and renewable bioplastic monomers.
Keyphrases
- escherichia coli
- poor prognosis
- dna methylation
- microbial community
- gene expression
- genome wide
- long non coding rna
- binding protein
- drinking water
- transcription factor
- climate change
- working memory
- klebsiella pneumoniae
- type diabetes
- risk assessment
- blood pressure
- cell wall
- pseudomonas aeruginosa
- staphylococcus aureus
- blood glucose