Humulus japonicus Extract Ameliorates Hepatic Steatosis Through the PPAR α -Mediated Suppression of Alcohol-Induced Oxidative Stress.
Wonjun ChoSeung Yeon ParkHeeseung OhA M Abd El-AtyAhmet HacimüftüoğluDae-Sung KimTae Woo JungJi Hoon JeongPublished in: Journal of medicinal food (2023)
Humulus japonicus has been used to treat obesity, hypertension, and nonalcoholic fatty liver and to alleviate inflammation and oxidative stress. In the present study, we aimed to investigate the effects of H. japonicus ethanol extracts (HE) and luteolin 7-O- β -d-glucoside (LU), which is identified as a major active component of H. japonicus , on ethanol-induced oxidative stress and lipid accumulation in primary hepatocytes. Mouse primary hepatocytes were treated with HE and stimulated with ethanol. The MTT test was used to determine cell viability. By using Western blotting, the effects of HE on the expression of different proteins were investigated. Experimental mice were given a 5% alcohol liquid Lieber-DeCarli diet to induce alcoholic fatty liver. We found that both HE and LU individually attenuated ethanol-induced lipid accumulation, lipogenic protein expression, and cellular oxidative stress in hepatocytes. Treatment with HE or LU increased PPAR α and SOD1 expression and catalase activity in a dose-dependent manner. Small interfering RNA of PPAR α reduced the effects of HE on oxidative stress, lipid metabolism, and levels of antioxidants. We also observed that orally administered HE treatment alleviated hepatic steatosis in a diet containing ethanol-fed mice. This study suggests HE as a functional food that can improve hepatic steatosis, thereby preventing hepatic injury caused by alcohol consumption.
Keyphrases
- oxidative stress
- alcohol consumption
- diabetic rats
- insulin resistance
- high fat diet induced
- liver injury
- weight loss
- dna damage
- fatty acid
- poor prognosis
- drug induced
- ischemia reperfusion injury
- physical activity
- blood pressure
- hydrogen peroxide
- induced apoptosis
- metabolic syndrome
- type diabetes
- south africa
- binding protein
- mouse model
- heat shock
- long non coding rna
- nitric oxide
- human health
- high resolution
- atomic force microscopy
- replacement therapy
- endothelial cells
- ionic liquid
- endoplasmic reticulum stress