Ozone-Based Eye Drops Activity on Ocular Epithelial Cells and Potential Pathogens Infecting the Front of the Eye.
Roman PaduchTeresa Urbanik-SypniewskaJolanta KutkowskaTomasz ChorągiewiczAnna Matysik-WoźniakSandrine Anne ZweifelAleksandra Czarnek-ChudzikWojciech ZałuskaRobert RejdakMario Domenico ToroPublished in: Antioxidants (Basel, Switzerland) (2021)
Confirmation of the biological effectiveness of new ophthalmic preparations introduced in the market is an important element in maintaining the safety of using this type of medications. This study aimed to investigate the activity of Ozodrop® on human corneal and conjunctival epithelial cells, as well as its antibacterial and antifungal activity. Cytotoxicity analyses of ocular surface epithelial cells were performed in vitro by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) and Neutral Red uptake assays. The level of nitric oxide released by the cells was assessed by the Griess method. The reduction of the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical by the tested formulation was analyzed. Microbiological tests were also performed. It was found that the Ozodrop® preparation exhibited biological activity, but was less active than the reference antibiotics and the anti-yeast agent. The cytotoxic activity of the Ozodrop® formulation was dependent on the time of cell exposure to it. No toxic effect was observed in the short-term, for up to 3 h. It appeared after 24 h of exposure of the cells to the preparation. The drops showed antioxidant activity in the specified concentration range. They also stimulated the release of nitric oxide, mainly by corneal epithelial cells. The Ozodrop® formulation exhibits biological activity that can be considered useful in the treatment of infections in the front part of the eye.
Keyphrases
- nitric oxide
- induced apoptosis
- drug delivery
- cell cycle arrest
- hydrogen peroxide
- randomized controlled trial
- endothelial cells
- systematic review
- wound healing
- optical coherence tomography
- nitric oxide synthase
- endoplasmic reticulum stress
- oxidative stress
- high throughput
- health insurance
- stem cells
- particulate matter
- cell therapy
- mass spectrometry
- pluripotent stem cells
- climate change
- antimicrobial resistance
- combination therapy
- liquid chromatography