SBA-15 Mesoporous Silica as Delivery Vehicle for rhBMP-2 Bone Morphogenic Protein for Dental Applications.
Dimitrios GkiliopoulosIoannis TsamesidisAnna TheocharidouGeorgia K PouroutzidouEvi ChristodoulouEvangelia StalikaKonstantinos XanthopoulosDimitrios N BikiarisKonstantinos S TriantafyllidisEleana KontonasakiPublished in: Nanomaterials (Basel, Switzerland) (2022)
(1) Background: A proposed approach to promote periodontal tissue regeneration in cases of peri-implantitis is the local administration of growth factors at the implant site. Recombinant human bone morphogenetic protein-2 (rh-BMP-2) can effectively promote bone regeneration and osseointegration and the development of appropriate carriers for its delivery is of paramount importance. The aim of the present study was to develop SBA-15 mesoporous nanoparticles (MSNs) with varying porosity, evaluate their biocompatibility with human Periodontal Ligament Cells (hPDLCs) and to investigate their effectiveness as carriers of rh-BMP-2. (2) Methods: SBA-15 type mesoporous silicas were synthesized via sol-gel reaction. The calcined SBA-15 samples were characterized by N 2 porosimetry, Fourier transform-infrared spectrometry (FTIR), Scanning (SEM) and Transmission Electron Microscopy (TEM). Rh-BMP-2 loading and release kinetics were evaluated by UV spectroscopy. (3) Results: MSNs presented hexagonally arranged, tubular pores of varying length and diameter. Slightly higher loading capacity was achieved for SBA-15 with large pores that presented good hemocompatibility. MTT assay revealed no cytotoxic effects for all the tested materials, while SBA-15 with large pores induced a significant upregulation of cell viability at day 5. (4) Conclusions: SBA-15 MSNs may prove a valuable delivery platform towards the effective release of bone-inducing proteins.
Keyphrases
- bone regeneration
- electron microscopy
- recombinant human
- mesenchymal stem cells
- high resolution
- high throughput
- endothelial cells
- stem cells
- randomized controlled trial
- high glucose
- soft tissue
- induced apoptosis
- systematic review
- mass spectrometry
- oral health
- diabetic rats
- cell death
- amino acid
- metal organic framework
- endoplasmic reticulum stress
- cell cycle arrest
- poor prognosis
- bone loss
- protein protein
- optic nerve
- simultaneous determination