Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats.
Angelina SchindeleFabienne GehrkeCarla SchmidtSarah RöhrigAnnika DornHolger PuchtaPublished in: Nature communications (2022)
CRISPR/Cas has been mainly used for mutagenesis through the induction of double strand breaks (DSBs) within unique protein-coding genes. Using the SaCas9 nuclease to induce multiple DSBs in functional repetitive DNA of Arabidopsis thaliana, we can now show that cell death can be induced in a controlled way. This approach, named CRISPR-Kill, can be used as tool for tissue engineering. By simply exchanging the constitutive promoter of SaCas9 with cell type-specific promoters, it is possible to block organogenesis in Arabidopsis. By AP1-specific expression of CRISPR-Kill, we are able to restore the apetala1 phenotype and to specifically eliminate petals. In addition, by expressing CRISPR-Kill in root-specific pericycle cells, we are able to dramatically reduce the number and the length of lateral roots. In the future, the application of CRISPR-Kill may not only help to control development but could also be used to change the biochemical properties of plants.
Keyphrases
- crispr cas
- genome editing
- genome wide
- cell death
- transcription factor
- arabidopsis thaliana
- dna methylation
- tissue engineering
- cell cycle arrest
- induced apoptosis
- poor prognosis
- dna binding
- gene expression
- minimally invasive
- high frequency
- mesenchymal stem cells
- binding protein
- cell proliferation
- signaling pathway
- endoplasmic reticulum stress
- current status
- small molecule