A Novel Hydroxyapatite/Vitamin B 12 Nanoformula for Treatment of Bone Damage: Preparation, Characterization, and Anti-Arthritic, Anti-Inflammatory, and Antioxidant Activities in Chemically Induced Arthritic Rats.
Amany BelalRehab MahmoudEman E MohamedAhmed FarghaliFatma I Abo El-ElaAmr GamalFatma Mohamed HalfayaEsraa KhaledAbdelbasset A FarahatAhmed H E HassanMohammed M GhoneimMohamed TahaMohamed Y ZakyPublished in: Pharmaceuticals (Basel, Switzerland) (2023)
The usage of nanomaterials for rheumatoid arthritis (RA) treatment can improve bioavailability and enable selective targeting. The current study prepares and evaluates the in vivo biological effects of a novel hydroxyapatite/vitamin B 12 nanoformula in Complete Freund's adjuvant-induced arthritis in rats. The synthesized nanoformula was characterized using XRD, FTIR, BET analysis, HERTEM, SEM, particle size, and zeta potential. We synthesized pure HAP NPs with 71.01% loading weight percentages of Vit B12 and 49 mg/g loading capacity. Loading of vitamin B 12 on hydroxyapatite was modeled by Monte Carlo simulation. Anti-arthritic, anti-inflammatory, and antioxidant effects of the prepared nanoformula were assessed. Treated arthritic rats showed lower levels of RF and CRP, IL-1β, TNF-α, IL-17, and ADAMTS-5, but higher IL-4 and TIMP-3 levels. In addition, the prepared nanoformula increased GSH content and GST antioxidant activity while decreasing LPO levels. Furthermore, it reduced the expression of TGF-β mRNA. Histopathological examinations revealed an improvement in joint injuries through the reduction of inflammatory cell infiltration, cartilage deterioration, and bone damage caused by Complete Freund's adjuvant. These findings indicate that the anti-arthritic, antioxidant, and anti-inflammatory properties of the prepared nanoformula could be useful for the development of new anti-arthritic treatments.
Keyphrases
- anti inflammatory
- rheumatoid arthritis
- oxidative stress
- bone regeneration
- diabetic rats
- bone mineral density
- disease activity
- single cell
- high glucose
- early stage
- drug induced
- monte carlo
- poor prognosis
- ankylosing spondylitis
- physical activity
- body mass index
- bone marrow
- transforming growth factor
- body composition
- tissue engineering
- risk assessment
- lactic acid
- mass spectrometry
- bone loss
- endothelial cells
- soft tissue
- molecularly imprinted
- systemic lupus erythematosus
- extracellular matrix
- postmenopausal women
- weight gain
- mesenchymal stem cells
- idiopathic pulmonary fibrosis