Cancer Stem Cell-Targeted Gene Delivery Mediated by Aptamer-Decorated pH-Sensitive Nanoliposomes.
Parikshit MoitraSantosh Kumar MisraKrishan KumarPaturu KondaiahPhuong TranWei DuanSantanu BhattacharyaPublished in: ACS biomaterials science & engineering (2021)
A new pH-responsive cationic co-liposomal formulation was prepared in this study using the twin version of the amphiphile palmitoyl homocysteine, TPHC; natural zwitterionic lipid, DOPE; and cholesterol-based twin cationic lipid, C5C, at specified molar ratios. This co-liposome was further decorated with a newly designed fluorescently tagged, cholesterol-tethered EpCAM-targeting RNA aptamer for targeted gene delivery. This aptamer-guided nanoliposomal formulation, C5C/DOPE/TPHC at 8:24:1 molar ratio, could efficiently transport the genes in response to low pH of cellular endosomes selectively to the EpCAM overexpressing cancer stem cells. This particular observation was extended using siRNA against GFP to validate their transfection capabilities in response to EpCAM expression. Overall, the aptamer-guided nanoliposomal formulation was found to be an excellent transfectant for in vitro siRNA gene delivery.
Keyphrases
- cancer stem cells
- cancer therapy
- drug delivery
- gold nanoparticles
- sensitive detection
- circulating tumor cells
- cell adhesion
- magnetic nanoparticles
- reduced graphene oxide
- label free
- quantum dots
- poor prognosis
- low density lipoprotein
- fatty acid
- binding protein
- hyaluronic acid
- long non coding rna
- bioinformatics analysis