Comparative Analysis of Glycosylation Affecting Sensitization by Regulating the Cross-Reactivity of Parvalbumins in Turbot ( Scophthalmus maximus ), Conger Eel ( Conger myriaster ) and Sea Bass ( Micropterus salmoides ).

Parvalbumin (PV) is the most common allergen in fish. Some patients with fish allergy are allergic to only one species of fish but are tolerant to others; however, the underlying mechanism has not been identified. This study showed that three types of glycated fishes' PV showed a similar decrease in immunoglobulin E (IgE) binding. Glycosylation could improve the simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) digestion resistance of fishes' PV. We also discovered that the cross-reactivity between eel and turbot was weaker than that of bass; glycosylation can reduce cross-reactivity between eel/bass and turbot by downregulating Th2 cytokines and upregulating Th1 cytokines as well as downregulating the expression of G-T PV, G-E PV, G-B PV of IL-4 (94.31 ± 3.16, 73.26 ± 0.91, 94.95 ± 3.03 ng/mL), and IL-13 (38.84 ± 0.75, 33.77 ± 0.71, 36.51 ± 0.50 ng/mL) and upregulating the expression of IFN-γ (318.01 ± 3.46, 387.15 ± 3.30, 318.01 ± 4.21 ng/mL) compared with T PV, respectively. This study showed that glycosylation affected sensitization by regulating the cross-reactivity of parvalbumins.