A novel and efficient murine model of Bietti crystalline dystrophy.
Yafang WangYang LiuShu LiuXiaomeng LiXinxin LiuMing JiaoYuqin YangXueting LuoFenghua WangXiaoling WanXiaodong SunPublished in: Disease models & mechanisms (2022)
Bietti crystalline dystrophy (BCD) is an autosomal recessive inherited retinal disease, resulting in blindness in most patients. The etiology and development mechanism of it remain unclear. Given the defects in previous mouse models of BCD, we generated a new Cyp4v3-/- mouse model, using CRISPR/Cas9 technology, for investigating the pathogenesis of BCD. We estimated the ocular phenotypes by fundus imaging, optical coherence tomography (OCT) and full-field scotopic electroretinography, and investigated the histological features by Hematoxylin and Eosin staining, Oil Red O staining and immunofluorescence. This model effectively exhibited age-related progression that mimicked the human ocular phenotypes. Moreover, gas chromatography-mass spectrometry and RNA-seq analysis indicated that the defect of Cyp4v3 led to the abnormal lipid metabolism, inflammation activation and oxidative stress of retina. Notably, inflammation activation and oxidative stress could also promote the progression of BCD in light-induced retinal degeneration. In conclusion, our data provided evidence that we established a novel and more effective Cyp4v3 knockout preclinical mouse model for BCD, which served as a useful tool for evaluating the effect of drugs and gene therapy in vivo.
Keyphrases
- oxidative stress
- mouse model
- optical coherence tomography
- diabetic retinopathy
- optic nerve
- rna seq
- gene therapy
- gas chromatography mass spectrometry
- crispr cas
- end stage renal disease
- single cell
- early onset
- ischemia reperfusion injury
- induced apoptosis
- room temperature
- dna damage
- endothelial cells
- diabetic rats
- chronic kidney disease
- ejection fraction
- newly diagnosed
- high resolution
- electronic health record
- genome editing
- peritoneal dialysis
- prognostic factors
- big data
- fatty acid
- data analysis
- endoplasmic reticulum stress
- stem cells
- mass spectrometry
- heat shock
- patient reported outcomes
- bone marrow
- atomic force microscopy
- single molecule
- gas chromatography
- tandem mass spectrometry
- induced pluripotent stem cells
- high speed