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Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors.

Hans-Heinrich HoffmannWilliam M SchneiderFrancisco J Sánchez RiveraJoseph M LunaAlison W AshbrookYadira M Soto-FelicianoAndrew A LealJérémie Le PenInna Ricardo-LaxEleftherios MichailidisYuan HaoAnsgar F StenzelAvery PeaceC David AllisScott W LoweMargaret R MacDonaldJohn T PoirierCharles M Rice
Published in: bioRxiv : the preprint server for biology (2020)
Focused CRISPR screens targeting host factors in the SARS-CoV-2 interactome were performed for SARS-CoV-2, HCoV-229E, HCoV-NL63, and HCoV-OC43 coronaviruses.Focused interactome CRISPR screens achieve higher resolution compared to genome-wide screens, leading to the identification of critical factors missed by the latter.Parallel CRISPR screens against multiple coronaviruses uncover host factors and pathways with pan-coronavirus and virus-specific functional roles.The number of host proteins that interact with a viral bait protein is not proportional to the number of functional interactors.Novel SARS-CoV-2 host factors are expressed in relevant cell types in the human airway.
Keyphrases
  • sars cov
  • genome wide
  • dna methylation
  • respiratory syndrome coronavirus
  • copy number
  • crispr cas
  • high throughput
  • genome editing
  • endothelial cells
  • amino acid
  • binding protein
  • single molecule
  • bone marrow