Autologous humanized PDX modeling for immuno-oncology recapitulates features of the human tumor microenvironment.
Michael ChiorazziJan MartinekBradley KrasnickYunjiang ZhengKeenan J RobbinsRihao QuGabriel KaufmannZachary SkidmoreMelani JuricLaura A HenzeFrederic BröseckeAdam AdonyiJun ZhaoLiang ShanEsen SefikJacqueline MuddYe BiS Peter GoedegebuureMalachi GriffithObi GriffithAbimbola OyedejiSofia FertuzinhosRolando Garcia-MilianDaniel BoffaFrank DetterbeckAndrew DhanasoponJustin BlasbergBenjamin JudsonScott GettingerKaterina PolitiYuval KlugerKarolina PaluckaRyan C FieldsRichard A FlavellPublished in: Journal for immunotherapy of cancer (2023)
Humanization of the interleukin 6 locus in MISTRG6 mice enhances HSPC engraftment, making it feasible to model tumor-immune interactions in an autologous manner from a bedside bone marrow aspirate. The TME from these autologous tumors display hallmarks of the human TME including innate and adaptive immune activation and provide a platform for preclinical drug testing.