Functional characterization of human pluripotent stem cell-derived arterial endothelial cells.
Jue ZhangLi-Fang ChuZhonggang HouMichael P SchwartzTimothy HackerVernella VickermanScott SwansonNing LengBao Kim NguyenAngela ElwellJennifer BolinMatthew E BrownRon StewartWilliam J BurlinghamWilliam L MurphyJames A ThomsonPublished in: Proceedings of the National Academy of Sciences of the United States of America (2017)
Here, we report the derivation of arterial endothelial cells from human pluripotent stem cells that exhibit arterial-specific functions in vitro and in vivo. We combine single-cell RNA sequencing of embryonic mouse endothelial cells with an EFNB2-tdTomato/EPHB4-EGFP dual reporter human embryonic stem cell line to identify factors that regulate arterial endothelial cell specification. The resulting xeno-free protocol produces cells with gene expression profiles, oxygen consumption rates, nitric oxide production levels, shear stress responses, and TNFα-induced leukocyte adhesion rates characteristic of arterial endothelial cells. Arterial endothelial cells were robustly generated from multiple human embryonic and induced pluripotent stem cell lines and have potential applications for both disease modeling and regenerative medicine.
Keyphrases
- endothelial cells
- high glucose
- pluripotent stem cells
- single cell
- vascular endothelial growth factor
- nitric oxide
- randomized controlled trial
- rheumatoid arthritis
- cystic fibrosis
- crispr cas
- risk assessment
- oxidative stress
- dna methylation
- diabetic rats
- staphylococcus aureus
- gene expression
- peripheral blood
- copy number
- drug induced