Multiplex enCas12a screens detect functional buffering among paralogs otherwise masked in monogenic Cas9 knockout screens.

Merve DedeMegan McLaughlinEiru KimTraver Hart

Published in: Genome biology (2020)

Together, these observations strongly suggest that functionally redundant paralogs represent a targetable set of genetic dependencies that are systematically under-represented among cell-essential genes in monogenic CRISPR-based loss of function screens.

Keyphrases

genome wide
dna methylation
high throughput
copy number
crispr cas
genome editing
single cell
cell therapy
gene expression
stem cells