Fabrication and characterization of PLGA nanoparticles encapsulating large CRISPR-Cas9 plasmid.
Ami JoVeronica M Ringel-ScaiaDylan K McDanielCassidy A ThomasRui ZhangJudy S RiffleIrving Coy AllenRichey M DavisPublished in: Journal of nanobiotechnology (2020)
In this work, plasmids for the CRISPR-Cas9 system were encapsulated in nanoparticles comprised of PLGA and were shown to induce expression of bacterial Cas9 in murine bone marrow derived macrophages in vitro. These results suggest that this nanoparticle-based plasmid delivery method can be effective for future in vivo applications of the CRISPR-Cas9 system.