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Attempts for deriving extended pluripotent stem cells from common marmoset embryonic stem cells.

Sho YoshimatsuMayutaka NakajimaIki SonnRie NatsumeKenji SakimuraEna NakatsukasaToshikuni SasaokaMari NakamuraTakashi SerizawaTsukika SatoErika SasakiHongkui DengHideyuki Okano
Published in: Genes to cells : devoted to molecular & cellular mechanisms (2022)
Extended pluripotent stem cells (EPSCs) derived from mice and humans showed an enhanced potential for chimeric formation. By exploiting transcriptomic approaches, we assessed the differences in gene expression profile between extended EPSCs derived from mice and humans, and those newly derived from the common marmoset (marmoset; Callithrix jacchus). Although the marmoset EPSC-like cells displayed a unique colony morphology distinct from murine and human EPSCs, they displayed a pluripotent state akin to embryonic stem cells (ESCs), as confirmed by gene expression and immunocytochemical analyses of pluripotency markers and three-germ-layer differentiation assay. Importantly, the marmoset EPSC-like cells showed interspecies chimeric contribution to mouse embryos, such as E6.5 blastocysts in vitro and E6.5 epiblasts in vivo in mouse development. Also, we discovered that the perturbation of gene expression of the marmoset EPSC-like cells from the original ESCs resembled that of human EPSCs. Taken together, our multiple analyses evaluated the efficacy of the method for the derivation of marmoset EPSCs. This article is protected by copyright. All rights reserved.
Keyphrases
  • pluripotent stem cells
  • embryonic stem cells
  • gene expression
  • endothelial cells
  • dna methylation
  • cell therapy
  • high fat diet induced
  • high throughput
  • type diabetes
  • mesenchymal stem cells
  • human health