Geographical distribution and molecular survey of freshwater snail intermediate hosts of Schistosoma haematobium by DraI/Sh73 PCR in eliminated foci of Morocco.

Morocco had reached the level of schistosomiasis elimination 16 years ago. However the spread of freshwater snails in several breeding sites, and imported schistosome infection, still exist. Therefore, snail survey is a crucial component to sustain elimination progress. This study aimed to evaluate and to incorporate DraI/Sh73 PCR, for detecting early prepatent Schistosoma haematobium infection in snail host, into epidemiologic surveillance for schistosomiasis, particularly in reportedly eliminated foci where S.bovis overlaps with S. haematobium. The geographical distribution and the density of Bulinus truncatus and Planorbarius metidjensis were monitored for six years (2014-2019) and snail sampling were conducted in Fkih Ben Saleh province. All snails were analyzed in pools by DraI/Sh73 PCR. Results showed absence of Planorbarius metidjensi and none of the collected Bulinus truncatus snails were infected by S. haematobium . DraI/Sh73 PCR using pooled snail extracts is specific, feasible and suitable in routine malacological survey in the post elimination phase of schistosomiasis in Morocco.