Single Cerebral Organoid Mass Spectrometry of Cell-Specific Protein and Glycosphingolipid Traits.
Markéta NezvedováDurga JhaTereza VáňováDarshak GadaraHana KlímováJan RaškaLukáš OpálkaDáša BohačiakováZdenek SpacilPublished in: Analytical chemistry (2023)
Cerebral organoids are a prolific research topic and an emerging model system for neurological diseases in human neurobiology. However, the batch-to-batch reproducibility of current cultivation protocols is challenging and thus requires a high-throughput methodology to comprehensively characterize cerebral organoid cytoarchitecture and neural development. We report a mass spectrometry-based protocol to quantify neural tissue cell markers, cell surface lipids, and housekeeping proteins in a single organoid. Profiled traits probe the development of neural stem cells, radial glial cells, neurons, and astrocytes. We assessed the cell population heterogeneity in individually profiled organoids in the early and late neurogenesis stages. Here, we present a unifying view of cell-type specificity of profiled protein and lipid traits in neural tissue. Our workflow characterizes the cytoarchitecture, differentiation stage, and batch cultivation variation on an individual cerebral organoid level.
Keyphrases
- single cell
- mass spectrometry
- subarachnoid hemorrhage
- high throughput
- neural stem cells
- cerebral ischemia
- cell therapy
- genome wide
- liquid chromatography
- randomized controlled trial
- cell surface
- induced pluripotent stem cells
- endothelial cells
- spinal cord
- induced apoptosis
- stem cells
- gene expression
- binding protein
- fatty acid
- cerebral blood flow
- small molecule
- ms ms
- signaling pathway
- quantum dots
- spinal cord injury
- cell proliferation
- fluorescent probe