Identifying miRNA-mRNA regulatory networks on extreme n-6/n-3 polyunsaturated fatty acid ratio expression profiles in porcine skeletal muscle.
Yron Joseph Yabut ManaigLourdes Criado-MesasAnna Esteve-CodinaEmilio Marmol-SanchezAnna CastellóArmand SánchezJosep M FolchPublished in: PloS one (2023)
Omega-3 (n-3) and omega-6 (n-6) polyunsaturated fatty acids (PUFAs) are essential fatty acids with antagonistic inflammatory functions that play vital roles in metabolic health and immune response. Current commercial swine diets tend to over-supplement with n-6 PUFAs, which may increase the likelihood of developing inflammatory diseases and affect the overall well-being of the animals. However, it is still poorly understood how n-6/n-3 PUFA ratios affect the porcine transcriptome expression and how messenger RNAs (mRNAs) and microRNAs (miRNAs) might regulate biological processes related to PUFA metabolism. On account of this, we selected a total of 20 Iberian × Duroc crossbred pigs with extreme values for n-6/n-3 FA ratio (10 high vs 10 low), and longissimus dorsi muscle samples were used to identify differentially expressed mRNAs and miRNAs. The observed differentially expressed mRNAs were associated to biological pathways related to muscle growth and immunomodulation, while the differentially expressed microRNAs (ssc-miR-30a-3p, ssc-miR-30e-3p, ssc-miR-15b and ssc-miR-7142-3p) were correlated to adipogenesis and immunity. Relevant miRNA-to-mRNA regulatory networks were also predicted (i.e., mir15b to ARRDC3; mir-7142-3p to METTL21C), and linked to lipolysis, obesity, myogenesis, and protein degradation. The n-6/n-3 PUFA ratio differences in pig skeletal muscle revealed genes, miRNAs and enriched pathways involved in lipid metabolism, cell proliferation and inflammation.
Keyphrases
- fatty acid
- skeletal muscle
- insulin resistance
- cell proliferation
- oxidative stress
- immune response
- binding protein
- single cell
- weight loss
- genome wide analysis
- climate change
- adipose tissue
- high fat diet induced
- transcription factor
- public health
- healthcare
- poor prognosis
- genome wide
- gene expression
- long non coding rna
- rna seq
- metabolic syndrome
- long noncoding rna
- cell cycle
- dendritic cells
- mental health
- physical activity
- weight gain
- toll like receptor
- body mass index
- breast reconstruction
- bioinformatics analysis