Generation of multidrug resistant human tissues by overexpression of the ABCG2 multidrug transporter in embryonic stem cells.
Zsuzsa ErdeiAnita SchambergerGyörgy TörökKornélia SzebényiGyörgy VáradyTamás I OrbánLászló HomolyaBalázs SarkadiÁgota ApátiPublished in: PloS one (2018)
The ABCG2 multidrug transporter provides resistance against various endo- and xenobiotics, and protects the stem cells against toxins and stress conditions. We have shown earlier that a GFP-tagged version of ABCG2 is fully functional and may be used to follow the expression, localization and function of this transporter in living cells. In the present work we have overexpressed GFP-ABCG2, driven by a constitutive (CAG) promoter, in HUES9 human embryonic stem cells. Stem cell clones were generated to express the wild-type and a substrate-mutant (R482G) GFP-ABCG2 variant, by using the Sleeping Beauty transposon system. We found that the stable overexpression of these transgenes did not change the pluripotency and growth properties of the stem cells, nor their differentiation capacity to hepatocytes or cardiomyocytes. ABCG2 overexpression provided increased toxin resistance in the stem cells, and protected the derived cardiomyocytes against doxorubicin toxicity. These studies document the potential of a stable ABCG2 expression for engineering toxin-resistant human pluripotent stem cells and selected stem cell derived tissues.
Keyphrases
- stem cells
- pluripotent stem cells
- embryonic stem cells
- endothelial cells
- multidrug resistant
- living cells
- wild type
- gene expression
- transcription factor
- poor prognosis
- escherichia coli
- cancer stem cells
- cell proliferation
- induced pluripotent stem cells
- drug resistant
- cell therapy
- fluorescent probe
- drug delivery
- oxidative stress
- high glucose
- binding protein
- risk assessment
- bone marrow
- cancer therapy
- heat stress