Assessing Larval Zebrafish Survival and Gene Expression Following Sodium Butyrate Exposure and Subsequent Lethal Bacterial Lipopolysaccharide (LPS) Endotoxin Challenge.
Mary X WangUmesh K ShandilyaXiang WuDavid HuybenNiel A KarrowPublished in: Toxins (2023)
As aquaculture production continues to grow, producers are looking for more sustainable methods to promote growth and increase fish health and survival. Butyrate is a short-chain fatty acid (SCFA) with considerable benefits to gut health, and in recent years, butyrate has been commonly used as an alternative to antimicrobials in livestock production. In this study, we aimed to assess the protective effects of sodium butyrate (NaB) on larval zebrafish subjected to a lethal Pseudomonas aeruginosa lipopolysaccharide (LPS) endotoxin challenge and to elucidate potential protective mechanisms of action. Larval zebrafish were pre-treated with 0, 3000, or 6000 μM NaB for 24 h at 72 h post-fertilization (hpf), then immune challenged for 24 h with 60 μg/mL of LPS at 96 hpf. Our results demonstrate that larval zebrafish pre-treated with 6000 μM of NaB prior to lethal LPS challenge experienced significantly increased survival by 40%, and this same level of NaB significantly down-regulated the expression of pro-inflammatory Tumor Necrosis Factor α ( TNF-alpha ). Findings from this study are consistent with the beneficial effects of NaB on other vertebrate species and support the potential use of NaB in aquaculture.
Keyphrases
- inflammatory response
- advanced non small cell lung cancer
- gene expression
- pseudomonas aeruginosa
- anti inflammatory
- healthcare
- aedes aegypti
- rheumatoid arthritis
- toll like receptor
- fatty acid
- drosophila melanogaster
- mental health
- free survival
- human health
- lps induced
- dna methylation
- poor prognosis
- epidermal growth factor receptor
- transcription factor
- escherichia coli
- climate change
- zika virus
- risk assessment
- newly diagnosed
- long non coding rna