Partial characterization of novel inulin-like prebiotic fructooligosaccharides of Sechium edule (Jacq.) Sw. (Cucurbitaceae) tuberous roots.

The fruits and aerial parts of Sechium edule (Jacq.) Sw. (Cucurbitaceae) are a popular cook vegetable being used in different parts of the world with ethnomedicinal and pharmacological values. However, the beneficial health attributes of the tuberous roots have been less exploited. The present study aimed to determine the prebiotic potentiality of the storage carbohydrates from this part. The carbohydrate fractions were harvested by hot-water, cold-water, hot-acid, hot-alkali, and hot 80% ethanol treatments following the standard protocol. The fractions were tested for in vitro prebiotic efficacy, hypocholesterolemic and antioxidant potentials, and in vivo health attributes in Swiss albino mice. The partial characterization was performed by high-performance thin-layer chromatography (HPTLC) and spectroscopic analyses by Fourier-transform infrared spectroscopy (FT-IR) and electrospray ionization mass spectrometry (ESI-MS). The highest prebiotic index was observed in hot-water and ethanol (Et-OH) fractions with the antioxidant IC50 values of 35.46 ± 0.33 and 32.56 ± 0.48 µg/ml, respectively. The HPTLC, FT-IR, and ESI-MS analyses showed that the hot-water and Et-OH carbohydrate fractions are rich in low-degree polymerizing inulin-like fructooligosaccharides (FOS). The fractions had a significant prebiotic index, hypocholesterolemic, and antioxidant activities. The synbiotic combination of the fractions with the probiotic LAB improved gut colonization and gut immune enhancement with significantly lowered triglycerides, serum LDL, and serum VLDL cholesterols. A significantly enhanced HDL cholesterol level proves its health beneficial attributes comparable to the commercial inulin prebiotics. Thus, this plant's novel inulin-like FOS may substitute the high-cost commercial prebiotics for our daily life. PRACTICAL APPLICATIONS: Prebiotics are low-degree polymerizing oligosaccharide food ingredients having multifaceted health benefits. For this reason, there is an ever-increasing global demand for such novel prebiotics. Therefore, finding out some novel prebiotic from conventional food sources may provide an alternative dietary source to fulfill the consumer demand. Sechium edule is a famous cook vegetable used by many ethnic communities across the globe. For the first time, the study revealed novel inulin-like fructooligosaccharides in the tuberous roots having hypocholesterolemic and synbiotic efficacy with GRAS lactic acid bacteria.