Association of KCTD15 gene with fat deposition in pigs.
Pan ZhangYu FuRan ZhangPeng ShangHao ZhangHao ZhangPublished in: Journal of animal physiology and animal nutrition (2021)
KCTD15 is associated with body mass index and fat deposition in humans, mice and chickens. However, the function of KCTD15 in pig fat deposition remains unclear. In this study, we cloned and analysed the cDNA sequence of porcine KCTD15. The full length of the mRNA sequence of KCTD15 is 4,091 bp, encoding 283 amino acids. The protein is hydrophilic, it has a relative molecular mass of about 31.9 kDa and an isoelectric point of 7.09 with no signal peptide sequence or transmembrane structure. Expression analysis showed that KCTD15 expression level was significantly higher in the tissues of Large White pigs (LW) than in those of Tibetan pigs (TP) and Diannan Small-ear pigs (DN) at 6 months of age, whereas its expression level in embryonic tissues of LW at 60 days was lower than that in tissues of TP and Wujin pigs (WJ). In pig primary adipocytes, the expression level of KCTD15 is high in the early stage of differentiation and gradually decreases in later stages. Additionally, the single-nucleotide polymorphism (SNP) site T-2030C (T/C mutation, located 2,030 bp upstream of the start codon) showed a dominant allele T with high promoter activity in the LW population and a dominant allele C in the TP and WJ populations. Our results indicate that KCTD15 is involved in pig fat deposition and that T-2030C is an important regulatory site for transcriptional activity, affecting fat deposition.
Keyphrases
- adipose tissue
- poor prognosis
- gene expression
- amino acid
- early stage
- binding protein
- fatty acid
- transcription factor
- genome wide
- long non coding rna
- radiation therapy
- type diabetes
- copy number
- metabolic syndrome
- high resolution
- oxidative stress
- mass spectrometry
- heat stress
- genome wide identification
- skeletal muscle
- sentinel lymph node