Antarctic krill oil exhibited synergistic effects with nobiletin and theanine on regulating ligand-specific receptor-mediated transcytosis in blood-brain barrier by inhibiting alkaline phosphatase in SAMP8 mice.

Blood brain barrier (BBB) impairment was related to the development of Alzheimer's disease (AD), which was dependent not only on tight junction but also on transcytosis of brain endothelial cells (BECs) in BBB. Aging induced the decrease of ligand-specific receptor-mediated transcytosis (RMT) and the increase of non-specific caveolar transcytosis in BECs, which led to the entry into parenchyma of neurotoxic proteins and the smaller therapeutic index in central nervous system drug delivery, further provoke neurodegenerative disease. Our previous study suggested that sea-derived Antarctic krill oil (AKO) exhibited synergistic effects with land-derived nobiletin (NOB) and theanine (THE) on ameliorating memory and cognitive deficiency in SAMP8 mice, however, it is still unclear whether BBB change was involved in. Hence, it was investigated that the effects of AKO combined with NOB and THE on aging-induced BBB impairment, including tight junction between BECs, ligand-specific RMT and non-specific caveolar transcytosis in BECs. The results suggested that AKO exhibited synergistic effects with NOB and THE on regulating ligand-specific RMT in BBB by inhibiting alkaline phosphatase (ALPL). The study provided a potential strategy candidate or targeted dietary patterns to prevent and treat AD by improving the BBB function. This article is protected by copyright. All rights reserved.