Protective Effect of Polyphenols Purified from Mallotus oblongfolius on Ethanol-Induced Gastric Mucosal Injury by Regulating Nrf2 and MAPKs Pathways.
Shasha YuZhouwei DuanPeng LiShiping WangLijun GuoGuang-Hua XiaHui XiePublished in: Antioxidants (Basel, Switzerland) (2022)
Mallotus oblongifolius (MO), which is rich in polyphenols, is a characteristic tea resource with medicinal value. In this study, a total of 45 polyphenolic components of MO, including narirutin, isoquercitrin, rutin and digallic acid, were identified by UPLC-Q-TOF/MS analysis. In addition, the gastroprotective effect of Mallotus oblongifolius polyphenols (MOP) on ethanol-induced gastric mucosal injury in rats was investigated. The rats received anhydrous ethanol after continuous gavage of MOP or lansoprazole for one week. In addition, the macro- and micro-damage induced by ethanol in the gastric tissue was significantly reduced after MOP pretreatment for one week. Further analysis showed that MOP prevented ethanol-induced acute gastric mucosal injury by increasing the expression of antioxidant enzymes (SOD, CAT, GSH-Px) and decreasing the expression of reactive oxygen species (ROS), lipid oxidation product (MDA) and myeloperoxidase (MPO). Meanwhile, MOP inhibited the phosphorylation of p38/ERK/JNK and promoted the activation of the Nrf2 pathway. These results suggested that MOP may be a promising therapeutic target for the prevention of ethanol-induced gastric mucosal injury by improving oxidative stress, inhibiting the p38/ERK/JNK signaling pathways and activating Nrf2 expression.
Keyphrases
- oxidative stress
- signaling pathway
- diabetic rats
- poor prognosis
- induced apoptosis
- reactive oxygen species
- high glucose
- pi k akt
- dna damage
- cell death
- ulcerative colitis
- cell proliferation
- epithelial mesenchymal transition
- drug induced
- ischemia reperfusion injury
- binding protein
- long non coding rna
- endothelial cells
- clinical trial
- fatty acid
- high resolution
- heat shock
- protein kinase