Aerosol Jet Printing-Enabled Dual-Function Electrochemical and Colorimetric Biosensor for SARS-CoV-2 Detection.
Li LiuZhiheng XuAdrian Moises Molina VargasStephen J DolleryMichael G SchrlauDenis CormierMitchell R O'ConnellGregory J TobinKe DuPublished in: Analytical chemistry (2023)
An aerosol jet printing-enabled dual-function biosensor for the sensitive detection of pathogens using SARS-CoV-2 RNA as an example has been developed. A CRISPR-Cas13:guide-RNA complex is activated in the presence of a target RNA, leading to the collateral trans-cleavage of ssRNA probes that contain a horseradish peroxidase (HRP) tag. This, in turn, catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by HRP, resulting in a color change and electrochemical signal change. The colorimetric and electrochemical sensing protocol does not require complicated target amplification and probe immobilization and exhibits a detection sensitivity in the femtomolar range. Additionally, our biosensor demonstrates a wide dynamic range of 5 orders of magnitude. This low-cost aerosol inkjet printing technique allows for an amplification-free and integrated dual-function biosensor platform, which operates at physiological temperature and is designed for simple, rapid, and accurate point-of-care (POC) diagnostics in either low-resource settings or hospitals.
Keyphrases
- sensitive detection
- label free
- loop mediated isothermal amplification
- sars cov
- gold nanoparticles
- quantum dots
- nucleic acid
- low cost
- crispr cas
- hydrogen peroxide
- living cells
- randomized controlled trial
- high frequency
- respiratory syndrome coronavirus
- water soluble
- ionic liquid
- healthcare
- small molecule
- genome editing
- high resolution
- high throughput
- single molecule
- nitric oxide
- antimicrobial resistance
- dna binding
- fluorescence imaging