Investigation of the Antifungal and Anticancer Effects of the Novel Synthesized Thiazolidinedione by Ion-Conductance Microscopy.
Nikita A SavinAlexander ErofeevRoman TimoshenkoAlexander N VaneevAnastasiia S GaraninaSergey SalikhovNatalia GrammatikovaIgor LevshinYuri KorchevPeter V GorelkinPublished in: Cells (2023)
In connection with the emergence of new pathogenic strains of Candida , the search for more effective antifungal drugs becomes a challenge. Part of the preclinical trials of such drugs can be carried out using the innovative ion-conductance microscopy (ICM) method, whose unique characteristics make it possible to study the biophysical characteristics of biological objects with high accuracy and low invasiveness. We conducted a study of a novel synthesized thiazolidinedione's antimicrobial (for Candida spp.) and anticancer properties (on samples of the human prostate cell line PC3), and its drug toxicity (on a sample of the human kidney cell line HEK293). We used a scanning ion-conductance microscope (SICM) to obtain the topography and mechanical properties of cells and an amperometric method using Pt-nanoelectrodes to register reactive oxygen species (ROS) expression. All data and results are obtained and presented for the first time.
Keyphrases
- candida albicans
- reactive oxygen species
- endothelial cells
- high resolution
- prostate cancer
- single molecule
- biofilm formation
- induced pluripotent stem cells
- pluripotent stem cells
- induced apoptosis
- escherichia coli
- high speed
- staphylococcus aureus
- poor prognosis
- oxidative stress
- optical coherence tomography
- cell death
- electronic health record
- emergency department
- hydrogen peroxide
- cell proliferation
- machine learning
- drug induced
- stem cells
- big data
- oxide nanoparticles
- binding protein