K 6 [P 2 Mo 18 O 62 ] as DNase-Mimetic Artificial Nucleases to Promote Extracellular Deoxyribonucleic Acid Degradation in Bacterial Biofilms.

In the current study, the DNase-like activity of the Dawson-type polyoxometalate K 6 [P 2 Mo 18 O 62 ] was explored. The obtained findings demonstrated that K 6 [P 2 Mo 18 O 62 ] could effectively cleave phosphoester bonds in the DNA model substrate (4-nitrophenyl phosphate) and result in the degradation of plasmid DNA. Moreover, the application potential of this Dawson-type polyoxometalate as a DNase-mimetic artificial enzyme to degrade extracellular DNA (eDNA) in Escherichia coli ( E. coli ) bacterial biofilm was explored. The results demonstrated that K 6 [P 2 Mo 18 O 62 ] exhibited high cleavage ability toward eDNA secreted by E. coli and thus eradicated the bacterial biofilm. In conclusion, Dawson-type polyoxometalate K 6 [P 2 Mo 18 O 62 ] possessed desirable DNase-like activity, which could serve as a bacterial biofilm eradication agent by cleaving and degrading eDNA molecules.