Cerebral cortex biopsies enable the investigation of native developing and mature human brain tissue. Here, we present a protocol to process human cortical biopsies from the surgical theater to the laboratory. We describe steps for the preparation of viable acute slices for patch-clamp recording using dedicated chemical solutions for transport and sectioning. We then explain procedures for tissue fixation and post hoc immunostaining to correlate physiological properties to morphological features and protein detection. For complete details on the use and execution of this protocol, please refer to Libé-Philippot et al. 1 .
Keyphrases
- endothelial cells
- randomized controlled trial
- subarachnoid hemorrhage
- induced pluripotent stem cells
- ultrasound guided
- functional connectivity
- pluripotent stem cells
- liver failure
- intensive care unit
- respiratory failure
- loop mediated isothermal amplification
- mass spectrometry
- amino acid
- high resolution
- blood brain barrier