Critical Evaluation of a microRNA-Based Risk Classifier Predicting Cancer-Specific Survival in Renal Cell Carcinoma with Tumor Thrombus of the Inferior Vena Cava.
Mischa J KotlyarMarkus KrebsAntonio Giovanni SolimandoAndré MarquardtMaximilian BurgerHubert KüblerRalf BargouSusanne KneitzWolfgang OttoJohannes BreyerDaniel C VerghoBurkhard KneitzCharis KalogirouPublished in: Cancers (2023)
(1) Background: Clear cell renal cell carcinoma extending into the inferior vena cava (ccRCC IVC ) represents a clinical high-risk setting. However, there is substantial heterogeneity within this patient subgroup regarding survival outcomes. Previously, members of our group developed a microRNA(miR)-based risk classifier-containing miR-21-5p, miR-126-3p and miR-221-3p expression-which significantly predicted the cancer-specific survival (CSS) of ccRCC IVC patients. (2) Methods: Examining a single-center cohort of tumor tissue from n = 56 patients with ccRCC IVC , we measured the expression levels of miR-21, miR-126, and miR-221 using qRT-PCR. The prognostic impact of clinicopathological parameters and miR expression were investigated via single-variable and multivariable Cox regression. Referring to the previously established risk classifier, we performed Kaplan-Meier analyses for single miR expression levels and the combined risk classifier. Cut-off values and weights within the risk classifier were taken from the previous study. (3) Results: miR-21 and miR-126 expression were significantly associated with lymphonodal status at the time of surgery, the development of metastasis during follow-up, and cancer-related death. In Kaplan-Meier analyses, miR-21 and miR-126 significantly impacted CSS in our cohort. Moreover, applying the miR-based risk classifier significantly stratified ccRCC IVC according to CSS. (4) Conclusions: In our retrospective analysis, we successfully validated the miR-based risk classifier within an independent ccRCC IVC cohort.
Keyphrases
- long non coding rna
- cell proliferation
- inferior vena cava
- poor prognosis
- long noncoding rna
- pulmonary embolism
- randomized controlled trial
- binding protein
- vena cava
- end stage renal disease
- newly diagnosed
- mass spectrometry
- prognostic factors
- high resolution
- ejection fraction
- papillary thyroid
- case report
- squamous cell carcinoma