Impact of Fecal Microbiota Transplantation on Gut Bacterial Bile Acid Metabolism in Humans.
Jessica-Miranda BustamanteTyson DawsonCaitlin LoefflerZara MarforiJulian R MarchesiBenjamin Harvey MullishChristopher C ThompsonKeith A CrandallAli RahnavardJessica R AllegrettiBethany P CummingsPublished in: Nutrients (2022)
Fecal microbiota transplantation (FMT) is a promising therapeutic modality for the treatment and prevention of metabolic disease. We previously conducted a double-blind, randomized, placebo-controlled pilot trial of FMT in obese metabolically healthy patients in which we found that FMT enhanced gut bacterial bile acid metabolism and delayed the development of impaired glucose tolerance relative to the placebo control group. Therefore, we conducted a secondary analysis of fecal samples collected from these patients to assess the potential gut microbial species contributing to the effect of FMT to improve metabolic health and increase gut bacterial bile acid metabolism. Fecal samples collected at baseline and after 4 weeks of FMT or placebo treatment underwent shotgun metagenomic analysis. Ultra-high-performance liquid chromatography-mass spectrometry was used to profile fecal bile acids. FMT-enriched bacteria that have been implicated in gut bile acid metabolism included Desulfovibrio fairfieldensis and Clostridium hylemonae . To identify candidate bacteria involved in gut microbial bile acid metabolism, we assessed correlations between bacterial species abundance and bile acid profile, with a focus on bile acid products of gut bacterial metabolism. Bacteroides ovatus and Phocaeicola dorei were positively correlated with unconjugated bile acids. Bifidobacterium adolescentis , Collinsella aerofaciens , and Faecalibacterium prausnitzii were positively correlated with secondary bile acids. Together, these data identify several candidate bacteria that may contribute to the metabolic benefits of FMT and gut bacterial bile acid metabolism that requires further functional validation.
Keyphrases
- end stage renal disease
- placebo controlled
- double blind
- mass spectrometry
- chronic kidney disease
- newly diagnosed
- healthcare
- peritoneal dialysis
- public health
- prognostic factors
- phase iii
- adipose tissue
- randomized controlled trial
- metabolic syndrome
- stem cells
- tandem mass spectrometry
- mental health
- high resolution
- machine learning
- squamous cell carcinoma
- ultra high performance liquid chromatography
- deep learning
- mesenchymal stem cells
- wastewater treatment
- high performance liquid chromatography
- big data
- health information
- risk assessment
- artificial intelligence
- patient reported
- rectal cancer
- human health
- data analysis
- gas chromatography