Transcription Factor EepR Is Required for Serratia marcescens Host Proinflammatory Response by Corneal Epithelial Cells.
Kimberly M BrothersStephen A K HarveyRobert M Q ShanksPublished in: Antibiotics (Basel, Switzerland) (2021)
Relatively little is known about how the corneal epithelium responds to vision-threatening bacteria from the Enterobacterales order. This study investigates the impact of Serratia marcescens on corneal epithelial cell host responses. We also investigate the role of a bacterial transcription factor EepR, which is a positive regulator of S. marcescens secretion of cytotoxic proteases and a hemolytic surfactant. We treated transcriptomic and metabolomic analysis of human corneal limbal epithelial cells with wild-type bacterial secretomes. Our results show increased expression of proinflammatory and lipid signaling molecules, while this is greatly altered in eepR mutant-treated corneal cells. Together, these data support the model that the S. marcescens transcription factor EepR is a key regulator of host-pathogen interactions, and is necessary to induce proinflammatory chemokines, cytokines, and lipids.
Keyphrases
- transcription factor
- optical coherence tomography
- wild type
- wound healing
- dna binding
- cataract surgery
- poor prognosis
- endothelial cells
- induced apoptosis
- machine learning
- electronic health record
- fatty acid
- candida albicans
- single cell
- cell cycle arrest
- big data
- long non coding rna
- binding protein
- newly diagnosed
- cell proliferation