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Efficient site-specific integration in CHO-K1 cells using CRISPR/Cas9-modified donors.

Mohammad Hassan KheirandishBehnaz RahmaniHossein Zarei JalianiFarzaneh BarkhordariMohammad Ali MazlomiFatemeh Davami
Published in: Molecular biology reports (2023)
Our study suggested reliable strategies for enhancing the CRISPR-mediated integration, introducing Chr3 pseudo-attP site as a potential candidate for the sustained transgene expression, which might be applied to promote the rCHO cell line development.
Keyphrases
  • crispr cas
  • genome editing
  • induced apoptosis
  • poor prognosis
  • cell cycle arrest
  • genome wide
  • endoplasmic reticulum stress
  • oxidative stress
  • signaling pathway
  • climate change
  • human health
  • risk assessment
  • dna methylation