Efficient site-specific integration in CHO-K1 cells using CRISPR/Cas9-modified donors.

Mohammad Hassan KheirandishBehnaz RahmaniHossein Zarei JalianiFarzaneh BarkhordariMohammad Ali MazlomiFatemeh Davami

Published in: Molecular biology reports (2023)

Our study suggested reliable strategies for enhancing the CRISPR-mediated integration, introducing Chr3 pseudo-attP site as a potential candidate for the sustained transgene expression, which might be applied to promote the rCHO cell line development.

Keyphrases

crispr cas
genome editing
induced apoptosis
poor prognosis
cell cycle arrest
genome wide
endoplasmic reticulum stress
oxidative stress
signaling pathway
climate change
human health
risk assessment
dna methylation