Efficient site-specific integration in CHO-K1 cells using CRISPR/Cas9-modified donors.
Mohammad Hassan KheirandishBehnaz RahmaniHossein Zarei JalianiFarzaneh BarkhordariMohammad Ali MazlomiFatemeh DavamiPublished in: Molecular biology reports (2023)
Our study suggested reliable strategies for enhancing the CRISPR-mediated integration, introducing Chr3 pseudo-attP site as a potential candidate for the sustained transgene expression, which might be applied to promote the rCHO cell line development.