Viral Vector-Based Transduction of Slice Cultures.
J Simon WiegertChristine Elizabeth GeeThomas G OertnerPublished in: Cold Spring Harbor protocols (2017)
Transgenes can be introduced into the cells of organotypic slice cultures using different delivery methods, such as biolistic transfection, electroporation, and viral vector-based transduction. These methods produce different patterns of transgene expression. Local injection of recombinant adeno-associated virus (rAAV) produces a small cluster of transgene-expressing neurons around the injection site. Expression in individual cells varies with the distance from the injection site, indicating that many neurons take up several rAAV particles. The serotype and promoter also play a role in transgene expression. Here, we present a protocol for the transduction of previously prepared hippocampal slice cultures with rAVV.
Keyphrases
- poor prognosis
- induced apoptosis
- cell cycle arrest
- sars cov
- spinal cord
- randomized controlled trial
- ultrasound guided
- binding protein
- long non coding rna
- gene expression
- transcription factor
- cell death
- oxidative stress
- spinal cord injury
- cell proliferation
- magnetic resonance
- zika virus
- dengue virus
- brain injury
- gene therapy
- cerebral ischemia